EPI X for printing intestinal cells

The human intestine plays a critical role in absorption of not only water and nutrients, but also pharmaceutical drugs. In addition to this primary function, it also serves as a barrier which protects the organism from pathogens. 3D printing of intestinal cells overcomes the limitations of classical 2D culture setup and gives rise to more advanced and specific tissue-like structures. To meet the needs of Your research, 3D printed constructs can be cultured in filter inserts which create a stabile platform for various studies, such as transport of nutrients and bioactive molecules, effect of pharmaceuticals or function of intestinal barrier.


The aim of this project was to evaluate the viability of Caco 2 cells 3D bioprinted in CELLINK® EPI X ink using the CELLINK® BIO X.


CELLINK Products Used

  • BIO X

Caco 2, Bright-field, 10x magnification

Cell Information

Caco2 is a cell line derived from human epithelial colorectal adenocarcinoma and it has been widely used as a model of the intestinal epithelial barrier.

This cell line has previously been used to study if components of the sigma B regulon, which controls the transcriptional response to stress, in Listeria monocytogenes contribute to cell invasion. It has also been to study the effect of maslinic acid, a promising chemopreventive agent, on colon cancer cell lines.

Cells can be printed in monoculture or in co-culture, even in separate layers or compartments. Use our BIO X 3D bioprinter and print up to three different types – in one go!

Printing Data

Caco2 cells were mixed with CELLINK EPI X bioink in 1:10 ratio and discs were printed with the BIO X.


  • Cell type: Caco2
  • Bioink: CELLINK EPI X
  • Concentration: 10 million cells /mL of bioink
  • 3D bioprinter: BIO X
  • 3D model: disc 3 x 0.6 mm
  • Nozzle diameter/gauge: 0.410 mm (22 G)
  • Speed: 10 mm/s
  • Pressure: 3-5 kPa


Cell number and viability in 3D bioprinted constructs remained stable between Day 1 and Day 8. All analysed samples exhibit viability over 80%. Stable cell number and high viability indicate the beneficial effect of the CELLINK EPI X bioink, which creates a suitable environment for cell growth and proliferation.