Ready to start to 3D Bioprinting? Before you do, take a look at these 5 tools that you need to get the process going:

  1. Traditional 3D printers are not suitable for bioprinting applications. Bioprinting is performed under much milder printing conditions compared to the printing of thermoplastics utilized in other additive manufacturing approaches. Additionally, a sterile environment must be maintain during the process to preserve cell viability. To successfully bioprint, a CELLINK Bio X Bioprinter or equivalent is necessary.

 

  1. You need a cartridge (or several cartridges, dependent on your construct complexity) of sterile bioink. Bioinks are the building blocks of a bioprinted construct, they are necessary for the fabrication of a bioprinted construct. It is important that the bioink you choose has suitable printability under the conditions you want to print.

 

  1. Once you have a bioprinter and sufficient bioinks it is necessary to instruct the printer how to print. To do this, you must generate a gcode file. A gcode file will provide instructions for the bioprinter regarding where to deposit bioink filaments to fabricate a construct. You can either write the gcode file yourself for simpler shapes or utilize a program such as Slic3r to generate a gcode file from complex computer models.

 

  1. If you are printing with cells you need to have your cell population suspended in cell media at the appropriate density for mixing into a bioink prior to printing. It is important that the cell density is sufficient to result in a cellularized construct. Often cell suspensions of several million cells per mL are utilized. Make sure you carefully mix your cells into the bioink to preserve viability, or you could use a CELLINK CELLMIXER kit for one step mixing.

 

  1. The final tool you need is the correct printing nozzle and pressure for your application. This is going to dependent on the resolution you want to print and the bioink you utilize. Consult your bioink printability sheets to find the correct parameters for printing. Ensure you are using the correct nozzle for your application or the resulting bioprinted construct may not be what you envisioned.  If your print is not what you envisioned, change your parameters and try again!