CELLINK A Series

CELLINK A is based on the naturally derived polysaccharide, alginate. Isolated from the cell walls of brown algae, alginate is a versatile biomaterial that’s used for diverse applications ranging from food to drug delivery. Alginates contain source dependent arrangement of (1-4)-linked-D-mannuronic acid (M units) and -L-guluronic acid (G units) monomers which comprise the alginate block polymer. The introduction of divalent cations such as Ca2+ bind G-units in adjacent chains together to rapidly form a gel. Alginates biocompatibility, ease of use, and versatility has resulted in the wide applicability of the material in many tissue engineering applications including bone and cartilage, along with cell and drug delivery. Additionally, the alginate can be readily modified to contain nearly any peptide sequence permitting the fabrication of cell-specific biomaterials to guide cell differentiation and tissue formation. Alginate is one of the most commonly bioinks being utilized in the bioprinting field, being utilized as a standalone bioink and as a base material for composite bioinks.

Printability Data

Conical tip (~23.4° C)

This diagram displays the printability behaviour and characteristics of CELLINK A, when being printed with a 25G conical tip at room temperature (approx. 23.4° C). The different lines correspond to different printing pressures, as indicated by the colors in the legend.

Lines

In order to assess printability one has to consider a lot of factors. Here we can see how CELLINK A performs when printing straight lines, using a printing pressure of 5 kPa.

Conical tip (~23.4° C)

This diagram displays the printability behaviour and characteristics of CELLINK A, when being printed with a 27G conical tip at room temperature (approx. 23.4° C). The different lines correspond to different printing pressures, as indicated by the colours in the legend.

Lines

In order to assess printability one has to consider a lot of factors. Here we can see how CELLINK A performs when printing straight lines, using a printing pressure of 5 kPa.

Customizations

CELLINK A-RGD

CELLINK A-RGD is one of our bioinks that has been optimized for enhanced cell adhesion. This bioink is based on the naturally derived polysaccharide, alginate, that is isolated from the cell walls of brown algae. Alginate is a versatile biomaterial that has been utilized in diverse applications ranging from food to drug delivery. Alginates contain source dependent arrangement of (1-4)-linked-D-mannuronic acid (M units) and -L-guluronic acid (G units) monomers which comprise the alginate block polymer. The introduction of divalent cations such as Ca2+ bind G-units in adjacent chains together to rapidly form a gel. However, it is an improved blend of the CELLINK A as it contains covalently conjugated L-Arginine-Glycine-L-Aspartic Acid peptide sequence, also known as RGD. This peptide sequence is derived from fibronectin and is the most commonly recognized sequence by cells for adhesion. RGD has wide applicability in the engineering of nearly every tissue. The sequence has also been utilized for the surfacing of implants such as bone and vascular implants to improve integration within the body.