CELLINK Bioink

CELLINK® Bioink was developed as the first universal bioink for utilization in any 3D bioprinting system. Combining the advantages of a shear thinning nanofibrillar cellulose and the versatility of alginate, CELLINK® Bioink exhibits unparalleled consistency in printability. Both components are derived from natural sources and under stringent quality control, ensuring excellent consistency across your research experiments. Beyond the excellent printability and cell viability, the bioink can be readily modified to contain a myriad of peptides and growth factors. Contact us for more information!

Printability Data

The chart to the right presents expected filament diameters versus translation rate and applied pressures for CELLINK Bioink at room temperature. These measurements were performed using a 22 gauge conical nozzle. This data should be used as a starting point for calibration of the material within your system. Layer height is equal to the nozzle diameter.

Here are example prints of parallel lines of CELLINK Bioink using a 22 G nozzle. The pressure was held constant while the translation rate was increased from 100 mm/min to 1000 mm/min left to right. Faster translation rates are recommended for more uniform lines. Pressure must be balanced with translation rate. Please contact us at support@cellink.com and we will be happy to help!

The chart to the right presents expected filament diameters versus translation rate and applied pressures for CELLINK Bioink at room temperature. These measurements were performed using a 25 gauge conical nozzle. This data should be used as a starting point for calibration of the material within your system. Layer height is equal to the nozzle diameter.

Here are example prints of parallel lines of CELLINK Bioink using a 25 G nozzle. The pressure was held constant while the translation rate was increased from 100 mm/min to 1000 mm/min left to right. Faster translation rates are recommended for more uniform lines. Pressure must be balanced with translation rate. Please contact us at support@cellink.com and we will be happy to help!

Customizations

CELLINK® RGD

CELLINK® RGD is an enhanced composition of our universal CELLINK® Bioink. This bioink contains a covalently conjugated L-Arginine-Glycine-L-Aspartic Acid peptide sequence, also known as RGD, while also maintaining an excellent printability similar to the CELLINK® bioink. This peptide sequence is derived from fibronectin and is the most commonly recognized sequence by cells for adhesion. RGD has wide applicability in the engineering of nearly every tissue. The sequence has also been utilized for the surface treatment of bone and vascular implants to improve integration within the body. Accelerate your research today with CELLINK® RGD! Our ready to print formulations come in several different RGD concentrations, inquire today!

CELLINK® FIBRIN

CELLINK® FIBRIN is an enriched composition of our universal CELLINK® Bioink developed for targeted cell differentiation. CELLINK® FIBRIN contains fibrinogen which is uniformly dispersed within the CELLINK® Bioink base to ensure consistent printability during the fabrication process. When your construct is crosslinked, by the thrombin and the ionic binding agent, it creates an interpenetrating fibrin network which will be the best environment for your cells to grow and proliferate. CELLINK® FIBRIN has a wide panel of uses, if you’re interested and want more details about it, don’t hesitate to contact us! And if you feel like significantly accelerating your research today then get onboard and let’s bioprint right now with CELLINK® FIBRIN!

CELLINK® SKIN

CELLINK® SKIN bioink incorporates fibrinogen to recreate the native wound-healing environment found in native tissue, particularly in skin. It is optimized for the culture of fibroblasts and keratinocytes utilized for the fabrication of skin constructs. CELLINK® SKIN comes with an enhanced crosslinking solution containing thrombin to permit development of a compound network with unparalleled stability. After crosslinking with thrombin, CELLINK® SKIN contains both in situ fibrin and fibrinogen to provide an environment resembling the native wound healing process.

CELLINK® BONE

CELLINK® BONE is a modified composition of our universal CELLINK® Bioink developed to guide cell differentiation and impart osteogenic capacity. Tricalcium phosphate particles are uniformly dispersed within the CELLINK® BONE which ensures consistent printability during the fabrication process. Accelerate your bone engineering research today! Inquire our CELLINK BONE.

CELLINK® LAMININKS

Laminins are proteins composed of three subunits referred to as a α-chain, β-chain and γ-chain. They are a major component of the basal lamina that lines the external surface of cell membranes. Its cross-like structure that serves as a foundation for many tissues. Laminins play an important role in cell differentiation, migration, adhesion and also involved in supporting healthy tissue. Several laminins have been identified, all of which have been related to different tissues and organs in the human body.

Our CELLINK® LAMININKS consist of five tissue-specific bioinks, LAMININK 111, LAMININK 121, LAMININK 411, LAMININK 521 and LAMININK+, that have been developed to improve maintenance and survival of tissue. The bioinks contain laminins to mimic the basal lamina and the natural tissue environment. These bioinks retain the excellent printability and crosslinking capability found with the universal CELLINK® Bioink.

CELLINK® LAMININK 111

Laminin 111 is an important protein during early development of the lining of tissues and organs. Specialized applications that this bioink is suited for include the culture of brain, kidney, liver or intestinal cells. In disease modeling, this bioink is relevant for studying Parkinson’s disease and cancer models.

CELLINK® LAMININK 121

CELLINK® LAMININK 121 can be utilized with several cell types and this bioink can be utilized as a great starting point for the culture of many cell types. Laminin 121 is widely expressed during embryonic and early development of the lining of tissues and organs. This laminin variation is also found predominantly in the placenta, skeletal muscle, and kidney tissue. CELLINK® LAMININK 121 is particularly suitable as a base material for the culture of several cell types, including skeletal muscle, brain, kidney and liver cells.

CELLINK® LAMININ 411

CELLINK® LAMININ 411 supports many different tissues types including cells from the pancreas, vascular, immune, nervous, hematopoietic systems. This bioink is suitable as a base material for the culture of several specific cell types, including pancreatic cells and neurons. The bioink may be utilized as a base material for the development of pancreas models and the study of brain and nerve tissue.

CELLINK® LAMININK 521

CELLINK® LAMININK 521 can be used as a great starting point for the culture of many cell types. Laminin 521 is the natural laminin for pluripotent and embryonic stem cells and therefore laminin 521 induces self-renewal of these cells. In addition, laminin 521 supports many diverse tissue cell types, such as cells from pancreas, vascular, nervous and muscular systems. Furthermore, the bioink can be used as a base for many specialized cells including hepatocytes, cardiomyocytes, and neurons.

CELLINK® LAMININK+

CELLINK® LAMININK+ is a blend of several types of laminins and retains the excellent printability and ease of crosslinking. CELLINK® LAMININK+ can be used as a great starting point for the culture of many cell types. Our revolutionary blend of laminins within this bioink has been optimized to maximize positive influence on cells.