Name: Dr. Behnam Noorani
Occupation: Biomedical Researcher
Institution: Texas Tech University
What are you currently using CELLINK technology for?
I am working at the Blood-Brain Barrier (BBB) laboratory (Ulrich Bickel MD) in which our research area encompasses pharmacokinetic studies to characterize drug transport at the blood-brain barrier level, and the development of novel drug targeting strategies to treat disorders of the central nervous system. One of the main obstacles in drug development of neuro-therapeutics is the presence of the BBB that restricts access to the brain for small and macro molecules. Therefore, advanced in-vitro models of the BBB can help to understand the physiology, in particular transport mechanism of BBB and drug development. We are using BIO X to fabricate microvascular networks as human BBB models via human brain microvascular endothelial cells (hBMEC) derived from iPSC based on 3D printing technique. Different markers and drugs will be used to measure the permeability of our model and the obtained values will be compared with other in-vitro models and in vivo estimates.
What sparked your interest to work with 3D Bioprinting?
Our final goal is to develop a reproducible in-vitro 3D model of the human BBB for physiological and pharmacological studies. Since organ-on a chip as a new class of in-vitro models have recently become popular as in-vitro models because of their superiority in recapitulating microenvironments compared with conventional in-vitro models, we hypothesized that using 3D printing to create a 3D model of BBB can reduce the gap between traditional in-vitro and in-vivo models with human physiology. 3D printing can allow us to print iPSC derived astrocytes, and human pericytes which make our model similar to human BBB.
What future projects are you hoping to use CELLINK technology for?
We are trying to use 3D printing technique with different hydrogels to create microvascular networks which have similar microenvironments and dynamic conditions. We will use BIO X to print different cell types to complete our model. 3D printed microvascular networks will be used as a model for permeability studies.
What do you find to be most exciting about working with 3D Bioprinting?
I believe the most exciting part of working with 3D printing is being able to create something from scratch to reality. I am sure organ printing will happen one day finally.