To go further in organ model development in 3D bioprinting, several bioink still need to be created. A conductive bioink was one of them. Achieving a physiological relevant conductivity for different use will open new possibilities for whole organ model development, such as nerve repair with new possibilities for tissular regeneration. Additionally, it can be used for muscular contraction models where after electric stimulation, cells can contract, leading to a functional muscle.
Developing a cell viable conductive bioink using GelMA base bioink, and single walled carbon nanotube to enhance conductivity.
A printing protocol of the GelMA based bioink has been optimized for both Inkredible+ and Bio X, equipped with a cooled print head. After a quick preparation, the bioink can be mixed with cells and printed with a high precision (0.35 mm). Afterwards, the bioink can be cross-linked with UV light without lowering the viability.
The conductivity is important to allow cells to communicate electrically to one another. Carbon nanotubes are remarkably conductive, leading to low concentrations, and no change in bioink behaviour.
The viability after printing, both Day 1 and Day 14, has been assessed for Human Dermal Fibroblasts (HDFs) and more sensitive Neural Progenitor Cells (NPCs). The viability for HDFs was at Day 1 80% and Day 14 at 95%. NPCs are like mentioned a sensitive cell type leading to the viability of Day 1 being 44% and at Day 14 22%. However, the cells tend to cluster which is not accounted for in the live/dead analysis.
In this study, the new CELLINK Conductive bioink demonstrate high precision while printing and a great conductivity had been achieved. It has also been shown that both HDF and NPC can be mixed in the bioink and cultured for 14 days. CELLINK Conductive ink is therefore appropriated to new models.
Stay tuned for more updates about our new CELLINK Conductive bioink.