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The Perriman Group bioink

The Perriman Group bioink

During the summer, PhD student Thomas Richardson from University of Bristol visited CELLINKs lab to manufacture and validate the Perriman Group bioink.

Aim

The aims of the collaboration were to evaluate the bioink for printability on the CELLINK INKREDIBLE+ and Bio X, as well as to test the suitability of the bioink for printing of different cell types.

Introduction to the group

Thomas Richardson is a PhD student with Dr. Adam Perriman at the University of Bristol, UK. He is one of a number of PhDs and postdocs working in 3D bioprinting, using the Perriman Group’s proprietary bioink and a custom modified 3D bioprinter. The Perriman group recently purchased a Bio X and INKREDIBLE+ and as a part of this ongoing collaboration, Thomas was sent to CELLINKs headquarters in Gothenburg to manufacture and validate the Perriman Group bioink

The Perriman Group bioink

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The Perriman group bioink is based on alginate and pluronic and functions by a dual-gelation mechanism, which results in a porous and mechanically stable print with excellent print fidelity. Work performed in the Perriman lab showed that stem cells could be grown in the bioink over several weeks and tissues such as cartilage and bone were generated.

Cell viability

In Gothenburg, several cell lines were bioprinted and viability was analysed after one and seven days. Some cell lines fared better than others, the most positive result came from the bioprinting of cancer cells which started to form spheroid-like structures after nine days. During bioprinting of grids with hepatic stellate cell, clear lines could be seen in the printing direction and the living cells also aligned in those lines as seen in the image beside where green cells are living and read are dead.

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Conclusion

The Perriman Group bioink displays good printability and a cell viability of over 60% in 3D printed structures with cancer cells. However, the bioink needs to be evaluated further in cell lines such as neurons and fibroblasts to increase cell viability.