EPI X for printing intestinal cellsThe human intestine plays a critical role in absorbing not just water and nutrients, but also pharmaceutical drugs. In addition to its primary function, it acts as a protective barrier against pathogens. 3D printing intestinal cells overcomes the limitations of traditional 2D cultures, enabling more advanced and specific tissue-like structures. To meet the needs of your research, 3D-printed constructs can be cultured in filter inserts to create a stable platform for studying nutrient transport and bioactive molecules, pharmaceutical effects and intestinal barrier function.
AimThe aim of this project was to evaluate the viability of Caco 2 cells 3D bioprinted in CELLINK® EPI X ink using the CELLINK® BIO X.
CELLINK products used
- CELLINK® EPI X
- BIO X
Cell InformationCaco2 is a cell line derived from human epithelial colorectal adenocarcinoma and it is used widely as a model of the intestinal-epithelial barrier.
This cell line has previously been used to study if components of the sigma B regulon, which controls the transcriptional response to stress, in Listeria monocytogenes contribute to cell invasion. It has also been used to study the effect of maslinic acid, a promising chemopreventive agent, on colon cancer cell lines.
Cells can be printed in monoculture or in coculture, in separate layers or compartments. Use our BIO X bioprinter and print up to three different types in one go!
Printing DataCaco2 cells were mixed with CELLINK EPI X bioink in 1:10 ratio and discs were printed with the BIO X.
- Cell type: Caco2
- Bioink: CELLINK EPI X
- Concentration: 10 million cells /mL of bioink
- 3D bioprinter: BIO X
- 3D model: disc 3 x 0.6 mm
- Nozzle diameter/gauge: 0.410 mm (22 G)
- Speed: 10 mm/s
- Pressure: 3-5 kPa