Customer Testimonial from L. Oliver & C. Salaud
``Developing a 3D biosphere model``Our customers, Dr. Lisa Oliver & Dr. Celine Salaud from University of Nantes in France are evaluating a 3D model of glioma cells and surrounding cancer-associated fibroblasts or tumor-activated fibroblasts. This is done using the CELLINK® LAMININK series to determine the role of the microenvironment on tumorigenesis. Read their testimonial here below.
CELLINK Products Used
- CELLINK® LAMININK series
Testimonial from L. Oliver & C. Salaud“The brain tumor is a complex and dynamic system consisting of various biochemical and mechanical cues that act in conjunction with its microenvironment. As such, a multicellular tumor in vitro model would be a powerful tool to resemble tumor pathophysiology.
We have attempted to develop a 3D biosphere model using Glioblastoma Multiforme patient-derived cultures (PDC) that would incorporate glioma cells with cognate cancer-associated fibroblasts (CAFs) or tumor-activated fibroblasts (TAFs). The effect of CAFs and/or TAFs on the proliferation, cell-cell interactions and response to treatment of PDC is being evaluated. We are using this model to reconstruct a simplified tumor using 3D bioprinting and then treating the tumor with a modified Stupp protocol (protocol used in clinic) to determine the role of the microenvironment on tumorigenesis. These types of experiments are being performed using the CELLINK® LAMININK series.”