Here at CELLINK, we want to inspire you to print more advanced models using the BIO X. In the following section, we demonstrate how to print two droplets on top of each other to create encapsulated droplets.
CELLINK products used
- CELLINK® RGD
- CELLINK® A
- BIO X
Drop on dropIn this video, we demonstrated printing encapsulated droplets composed of CELLINK RGD and CELLINK A. CELLINK RGD was diluted 1:10 with cell suspension prior to printing. To create a droplet core, CELLINK RGD was placed in the first printhead and a droplet was printed with a 25G nozzle. To encapsulate the droplet core, a CELLINK A cartridge with attached 20G nozzle was placed the second printhead and printed a droplet on-top of the CELLINK RGD droplet. Ionic crosslinking of CELLINK RGD core after bioprinting is optional in this application, since it is encapsulated by a thin layer of CELLINK A. Once the CELLINK A is crosslinked it creates a shell that prevents CELLINK RGD from dissolving.
Find the G-code for this droplet on droplet printing on Bioverse.
Less viscous CELLINK ACELLINK RGD is a viscous bioink and therefore makes a stable inner core. On the other hand, CELLINK A bioink has a lower viscosity, which makes it a good bioink for encapsulating the inner core (Figure 1). CELLINK A will form a shell around the printed CELLINK RGD droplet, creating a protective layer for the cells in CELLINK RGD.