Here at CELLINK, we want to inspire you to print more advanced models using the BIO X. In the following section, we demonstrate how to print a multi-layer grid using different bioinks for the infill and perimeter.
CELLINK products used
- CELLINK LAMININK 521.
- GelXA LAMININK 411.
- BIO X.
Create complex structuresIn this video, we demonstrate printing the perimeter and the infill of a multi-layer grid construct with two different bioinks. GelXA LAMININK 411 and CELLINK LAMININK 521 were diluted with 10% cell suspension and placed in the first and second pneumatic printhead, respectively. GelXA LAMININK 411 was used to print the perimeter through a 25G nozzle at 5 mm/sec and 30-35 kPa with a 650 ms preflow delay. CELLINK LAMININK 521 was used to print the infill through a 25G nozzle at 10 mm/sec and 15-20 kPa with a 300 ms preflow delay. The grids were printed on a 12-well plate lid for better visualization. Constructs printed with GelXA LAMININK 411 and CELLINK LAMININK 521 can be crosslinked by adding our crosslinking agent. After one medium wash, your cell laden constructs are ready to be cultured in standard cultivation environment.
The STL file for printing the grids in this video can be found here on Bioverse. To make this particular 10x10x0.75 mm grid choose 10% infill density and use a rectilinear grid pattern.