Printing three-layered grids
Here at CELLINK, we want to inspire you to print more advanced models using the BIO X. In the following section, we demonstrate how to print a three-layered grid with GelXA SKIN and build a skin model.
CELLINK products used
- GelXA SKIN
- BIO X
Printing GelXA with three printheadsIn this video, we demonstrate printing of three-layered grids with different infill densities using GelXA SKIN. The GelXA SKIN cartridges were placed in each of the three pneumatic printheads. All filaments were extruded through 22G nozzles at 5-15 kPa with 5 mm/sec on a Petri dish surface. Printhead 1 printed one layer with rectilinear pattern with infill density of 80%, printhead 2 printed two layers with rectilinear pattern with 20% infill density and finally, printhead 3 printed three grid layers with infill density of 10%. Printed layers of different infill density can represent three layers in a skin tissue model: epidermis, papillary dermis and reticular dermis, respectively. GelXA SKIN constructs can be crosslinked by adding our crosslinking agent enhanced with thrombin, which enzymatically converts fibrinogen component into fibrin, thus creating a stable network. After one medium wash, your cell laden droplets are ready to be cultured in standard cultivation environment. In addition, GelXA constructs can be also by photocrosslinked.
The G-code to this three-layered grid structure can be found on Bioverse!